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Image Search Results
Journal: Molecules and Cells
Article Title: Extracellular Vesicles-Encapsulated miR-153-3p Potentiate the Survival and Invasion of Lung Adenocarcinoma
doi: 10.14348/molcells.2022.2221
Figure Lengend Snippet: (A) qRT-PCR analysis of miR-153-3p expression in LUSC cell lines (YTMLC-90, NCI-H266, and SK-MES-1) and LUAD cell lines (NCI-H1993, SW1271 and NCI-H522). (B) TEM of EVs derived from LUAD cell lines: EVs/ NCI-H1993, EVs/SW1271, and EVs/NCI-H522. (C) Nanoparticle tracking analysis of EVs/NCI-H1993, EVs/SW1271, and EVs/NCI-H522. (D) Western blot analysis of exosome markers (CD63, TSG101, Alix, and Hsp90) and non-exosome markers (GRP94 and Cytochrome) in LUAD cells lysate and separated EVs. (E) qRT-PCR analysis of miR-153-3p expression in EVs derived from LUSC cell lines (YTMLC-90, NCI-H266, and SK-MES-1) and LUAD cell lines (NCI-H1993, SW1271, and NCI-H522). (F) Heat map of the significant differentially expressed miRNAs extracted from selected lung carcinomas cell populations (small cell lung carcinoma, adenocarcinoma, and squamous cell carcinoma) compared with adjacent normal tissues (GSE74190). The data in the figures are represented the mean ± SD. ** P < 0.01.
Article Snippet: Human LUAD cell lines (NCI-H1993, SW1271, NCI-H522) and
Techniques: Quantitative RT-PCR, Expressing, Derivative Assay, Western Blot
Journal: Molecules and Cells
Article Title: Extracellular Vesicles-Encapsulated miR-153-3p Potentiate the Survival and Invasion of Lung Adenocarcinoma
doi: 10.14348/molcells.2022.2221
Figure Lengend Snippet: (A) Venn diagrams of potential downstream targets of miR-153-3p in two databases (Diana and StarBase). (B) The predicted miR-153-3p binding sites in the BANCR transcript. (C) Relative luciferase activity of reporter containing the 3’ UTR of BANCR upon transfection with miR-152-3p mimics or mimics NC in LUAD cells. (D) qRT-PCR analysis of BANCR expression in NCI-H1993, SW1271, and NCI-H522 cells. (E) Overall survival of LUAD and LUSC patients in different expression of BANCR from GEPIA database. (F) qRT-PCR analysis of BANCR expression in NCI-H1993, SW1271, and NCI-H522 cells after transfection with miR-152-3p inhibitor. (G) Western blot analysis of p-pi3k, pi3k, p-akt, akt in the treatment with inh-miR-153-3p, BANCR-KD, or inh-miR-153-3p+BANCR-KD. (H) The cell viability was assessed at 1, 2, and 3 days after treatment with inh-miR-153-3p, BANCR-KD, and inh-miR-153-3p+BANCR-KD. (I) The cell viability was assessed at 1, 2, and 3 days after treatment with inh-miR-153-3p, BANCR-KD, inh-miR-153-3p+LY294002, or BANCR-KD+ LY294002. (J) Western blot analysis of E-cadherin and vimentin in the treatment with inh-miR-153-3p, BANCR-KD, or inh-miR-153-3p+BANCR-KD. (K) The relationship between BANCR and miR-153-3p was detected by StarBase. The data in the figures are represented the mean ± SD. * P < 0.05; ** P < 0.01.
Article Snippet: Human LUAD cell lines (NCI-H1993, SW1271, NCI-H522) and
Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Quantitative RT-PCR, Expressing, Western Blot